International researchers are looking into ways to promote better human health by improving gastrointestinal health, outlining their findings in the recently published ‘A core-shell multi-drug platform to improve gastrointestinal tract microbial health using 3D printing.’
3D printing is connected with the medical field today in countless ways as researchers and manufacturers continue to innovate, encouraged by the potential for an infinite number of designs to allow for medical models and instruments, devices such as implants and prosthetics, and accompanying software, hardware, and materials. In this study, the authors branch out into a more unique and complex area of medicine, creating core-shell structure fibers via electrohydrodynamic 3D printing.
These types of fibers can be fabricated in a variety of shapes and sizes, and have to ability to deliver and release drugs; in fact, the researchers state that the biphasic drug release profile in simulated intestinal fluid offers ‘a burst release within the first 12 h and a slower sustained release up to 72 h.’
Bacteria living in the gastrointestinal tract can be both good and bad. It may be responsible for providing vitamins, nutrients, and digestion, but in turn, can also be hazardous to human health when there is an imbalance—causing a range of health issues from obesity to diabetes, and even worse—cancer. With suitable probiotics, however, and resulting gastrointestinal health, many patients see their health improve overall.
The fibrous membranes used in this study were successful in showing the potential for improved gastrointestinal health, coupled with the electrohydrodynamic (EHD) 3D printing. The authors chose this technique due to the following advantages:
- Enabling of micro to nanoscale fiber engineering
- Control and development of complex structures
- Precise printing of stacked and aligned fibers
- Personalized drugs can be created
“… cellulose acetate (CA) and polyacrylic resin II (PRII) were used as excipient materials of enteric-specific medicine due to its good biocompatibility [29], nontoxicity [28], and pH dependent [30] characteristics,” explained the authors. “The CA encapsulating stachyose (CA-STA) was used as shell while PRII loading proteoglycan (PRII-PRO) was used to form the core of the fibrous structures.”
The EHD printer was comprised of:
- Programmable X-Y-Z movement stage
- High power voltage supply
- Two syringe pumps
- Coaxial stainless-steel nozzle (inner and outer needles)
For the study, the authors created a square grid, semi-circle, and a whole circle using 10-layer deposition with an average thickness of 61.5 ± 2.6 μm. The printed fibers were examined with optical microscopy, as the team replaced PRO with Rhodamine B into the loaded PRII-core layer. Diameters of the inner and outer shells were 10.8 ± 3.7 μm and 61.2 ± 6.2 μm.
The enteric capsule, tested a delivery system for oral drugs, was comprised of the sample fibers.
“The in vitro drug release was carried using PBS solution (pH = 7.4) to mimic intestinal fluid,” explained the researchers. “The LC for STA and PRO was 3.67 ± 0.1% and 1.1 ± 0.02%, respectively; and the EE was 90.3 ± 1.7% and 95.3 ± 0.5%, respectively. This confirms each drug was efficiently loaded into outer layer and inner layer, respectively.”
For each sample, thickness was measured before tensile testing was performed, noted respectively for the square, semi-circle, and whole-circle membranes at 64.4 ± 2.6 μm, 61.9 ± 4.2 μm, and 58.1 ± 2.5 μm.
Because zig-zag patterns were prominent in the membrane sandwich structure, the research team notes them as critical elements in deciding mechanical properties of the 3D printed samples. Tensile strengths and broken strains increased, however, as the circle proportion increased—attributed to circular fibers transforming into a straight line when under the pressure of tensile force. Maximum tensile strength was also noted as ‘greater than 0.22 MPa,’ the gastrointestinal pressure of in humans.
“The printed drug-loaded membrane possessed good biocompatibility on L929 cells and excellent improvement effects on Bifidobacterium bifidum, alongside adequate inhibition effects on E. coli. Compared to the control, the proliferation ratio of Bifidobacterium bifidum can be raised to 294.2% and 242.7%, respectively, at the treatment of 0.1 g PRII-STA/CA-PRO and PRII-PRO/CA-STA,” concluded the researchers.
“The inhibition effects on E. coli was 37.0% and 27.5%, at the treatment of 0.1 g PRII-STA/CA-PRO and PRII-PRO/CA-STA, respectively. This result confirmed the feasibility of improving proliferation of probiotics (Bifidobacterium bifidum) combining with inhibiting growth of pathogenic (E. coli) in a single dosage for using dual drug loading. The core–shell structures developed here can be extremely advantageous in efficient therapy for gastrointestinal conditions.”
What do you think of this news? Let us know your thoughts! Join the discussion of this and other 3D printing topics at 3DPrintBoard.com.
[Source / Images: ‘A core-shell multi-drug platform to improve gastrointestinal tract microbial health using 3D printing’]Subscribe to Our Email Newsletter
Stay up-to-date on all the latest news from the 3D printing industry and receive information and offers from third party vendors.
You May Also Like
Printing Money Episode 18: The DC Fly-In with Mark Burnham, AddMfgCoalition
It’s only been a week since the previous show, but Printing Money is back already with Episode 18. Certain events call for Printing Money’s coverage, and the recent 2nd Annual...
3DPOD Episode 199: Collaborative Design with Graham Bredemeyer, CEO of CADchat
About a decade ago, entrepreneur Graham Bredemeyer started Collider, a company that combined the best of 3D printing with injection molding. Now he runs CADChat, which hopes to make sharing...
Printing Money Episode 17: Recent 3D Printing Deals, with Alex Kingsbury
Printing Money is back with Episode 17! Our host, NewCap Partners‘ Danny Piper, is joined by Alex Kingsbury for this episode, so you can prepare yourself for smart coverage laced...
3DPOD Episode 198: High Speed Sintering with Neil Hopkinson, VP of AM at Stratasys
Neil Hopkinson, a pioneering 3D printing researcher, played a pivotal role in developing a body of research that is widely utilized today. He also invented High Speed Sintering (HSS), also...