AM Investment Strategies
AMS Spring 2023

Electrospun Scaffolds: Enhancements via 3D Printed Mesh Reinforcements

Formnext

Share this Article

In the recently published ‘3D printed mesh reinforcements enhance the mechanical properties of electrospun scaffolds,’ US researchers explore the use of tissue engineering structures for both regeneration and repair, using innovative materials to strengthen mechanical properties.

As research continues regarding scaffolding in clinical applications such as repair of the bladder or ligaments or grafting for bone or skin, scaffolds are being 3D printed and employed more often to mimic the extracellular matrix, scale up structures, and provide good cell adhesion and movement.

“To improve biocompatibility, methods have been developed to incorporate endogenous ECM molecules and/or growth factors to promote cell differentiation, survival, and/or proliferation,” state the researchers. “However, one of the major disadvantages of electrospun scaffolds is that they have relatively poor mechanical properties (low strength and stiffness, high ductility) compared to many of the tissues they are designed to heal. This limits their use in applications that require a material with relatively high mechanical strength and stiffness, such as bone or tendon repair.”

Scaffolds are often also used as composites, blended with synthetic polymers such as poly(ε-caprolactone) (PCL). This is a biodegradable, FDA-approved, well-established material. Other popular composites are:

  • poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)
  • poly (vinylidene fluoride) (PVD)
  • poly (lactic acid) (PLA)

“For many regenerative therapies, it is thought that the mechanical properties of an implanted scaffold should match that of the target tissue. Accordingly, new approaches are needed to enhance the mechanical properties of electrospun scaffolds to make them more suitable for applications in musculoskeletal repair and regeneration,” state the researchers.

PLA was used for printing directly onto electrospun scaffolds. The researchers noted that tensile strength and elastic modulus were increased, with ductility decreased, in using PLA meshes. They upheld their matrix structure after being strengthened with PLA, with no issues regarding rejection of implants when tested on rats. Two types of PLA were used—one with 6mm between the struts, and another with an 8mm distance between struts.

Fabrication of reinforced electrospun scaffolds. Electrospun scaffolds were produced from a 40:60 ratio of PCL:gelatin. The scaffolds were then placed in a 3D-printer and a PLA mesh was deposited onto one side of the scaffold. Two types of 3D-printed meshes were generated, one with a 6 mm distance between PLA struts, and the other with an 8 mm distance between struts

SEM imaging of reinforced electrospun scaffolds. a-c SEM images of the electrospun side of the reinforced scaffolds. Images show a uniform distribution of randomly oriented fibers. d-f SEM images of the 3D-printed side of the scaffolds. High magnification images (f) show that there is minimal damage to the electrospun fibers in the immediate vicinity of the 3D-printed PLA mesh. Yellow arrows depict the 3D-printed PLA. White arrowheads depict the PCL:gelatin scaffold

“When visualized under high magnification, it is apparent that deposition of the 3D printed layer did not compromise the woven structure of the electrospun scaffold. The electrospun fibers proximal to the PLA mesh were not melted from the heat of the 3D printing process,” stated the researchers.

Scaffolds were assessed by the research group as they used an in vivo bone graft model, which ultimately ‘suggested’ biocompatibility of the electrospun scaffolds. The scaffolds did not show a boundary separation between the electrospun and 3D printed layers; however, the mesh reinforcement was attached to the scaffold without interrupting the ‘architecture of the layer.’

“The addition of the 3D printed mesh is technically straightforward and can be applied to any type of electrospun scaffold, highlighting the adaptability of this approach to scaffolds of varying biochemical composition or structure,” concluded the researchers. “Furthermore, the differences in the mechanical properties imparted by the 6 mm vs. 8 mm reinforcements point to a potential strategy for tuning the strength and stiffness of electrospun scaffolds using meshes with different sizes and shapes.

“In sum, the current investigation suggests that the mechanical properties of electrospun scaffolds can be markedly improved by the addition of tunable 3D printed meshes, while preserving the desirable aspects of the electrospun material.”

Scaffolding in 3D printing is an area of fabrication that just continues to grow, especially in the areas of tissue regeneration, bone replacement, cartilage regrowth, and more.

What do you think of this news? Let us know your thoughts! Join the discussion of this and other 3D printing topics at 3DPrintBoard.com.

[Source / Images: ‘3D printed mesh reinforcements enhance the mechanical properties of electrospun scaffolds’]

Share this Article


Recent News

3D Printing News Unpeeled: HP, Autodesk, Azure Homes, Fathom and Mantle

3D Printing Firm 6K to Make Battery Materials with ONE



Categories

3D Design

3D Printed Art

3D Printed Food

3D Printed Guns


You May Also Like

Battery 3D Printing Startup Sakuu Secures Japanese Spark Plug Leader for Ceramic Materials

As Bay Area startup Sakuu continues toward commercialization of its solid state battery (SSB) 3D printing technology, the company has secured a partnership with NGK Spark Plugs (TYO: 5334), a...

3D Printing News Unpeeled: Rocket Lab, Sierra Space, Caracol, 6K

We explore Rocket Lab and Sierra Space join alliance to help move troops and material via rockets for terrestrial transport, Sandhelden and Duffy London 3D printing a sand coffee table, Caracol...

Incodema3D Signals US Metal 3D Printing Scale-Up with 6K, Uniformity Deals

Incodema3D, an additive manufacturing (AM) services company specializing in the aerospace sector, announced two new projects recently, involving two different metal alloy powders. First, Incodema3D, which is headquartered in upstate...

UCLA Materials Scientists Awarded Grant for 3D Printed Batteries

The University of California, Los Angeles (UCLA) announced that a team of materials scientists at the university’s Samueli School of Engineering has received a grant to develop a new additive...