Researchers Develop New 3D Imaging Method as a More Cost-Effective, Accurate Way to Visualize Cells in Tumors

Share this Article

Advanced 3D imaging has been used often in the medical field, for applications as varied as to study the internal body structure of humans, prove if genes can influence our facial appearance, and even provide earlier breast cancer detection. A team of researchers with the National Institute of Allergy and Infectious Diseases (NIAID), one of the National Institutes of Health (NIH), have been hard at work developing a new, detailed method of 3D visualizing how cell types are distributed in complex tissues, such as tumors, called Clearing-Enhanced 3D microscopy (Ce3D).

Chemical tissue clearing techniques are used by hospital laboratories and researchers to make dense, opaque tissue transparent when they need detailed images of tissue samples, especially from tumor cells, which then lets scientists use state-of-the-art microscopes to identify and figure out the distribution of certain cells that could be cancerous and have already been marked with easy-to-see antibodies or fluorescent compounds. However, current tissue clearing techniques are limited and hard to use, so researchers have a difficult time understanding how a patient’s immune system is affecting the tumor. Some scientists use methods other than tissue clearing to count the cell types in tissues, but these techniques mean grinding up or slicing the tissue samples.

This new 3D tissue imaging technique could help medical researchers provide evaluations of how well various immunotherapies are targeting complex cancers. By using Ce3D, researchers are able to keep the biopsied tissue in one piece, use tracking methods to mark many different cell types, and then view the results in accurate 3D.

Ce3D allows scientists to “clear” tissues for easier analysis.

Ronald N. Germain, MD, PhD, and chief of the Laboratory of Systems Biology in NIAID’s Division of Intramural Research, together with Weizhe Li, PhD, also from NIAID’s Laboratory of Systems Biology and Michael Y. Gerner, PhD, with the University of Washington’s Department of Immunology, published a paper on their findings, titled “Multiplex, quantitative cellular analysis in large tissue volumes with clearing-enhanced 3D microscopy (Ce3D),” in Proceedings of the National Academy of Sciences.

The abstract reads, “Organ homeostasis, cellular differentiation, signal relay, and in situ function all depend on the spatial organization of cells in complex tissues. For this reason, comprehensive, high-resolution mapping of cell positioning, phenotypic identity, and functional state in the context of macroscale tissue structure is critical to a deeper understanding of diverse biological processes. Here we report an easy to use method, clearing-enhanced 3D (Ce3D), which generates excellent tissue transparency for most organs, preserves cellular morphology and protein fluorescence, and is robustly compatible with antibody-based immunolabeling. This enhanced signal quality and capacity for extensive probe multiplexing permits quantitative analysis of distinct, highly intermixed cell populations in intact Ce3D-treated tissues via 3D histo-cytometry. We use this technology to demonstrate large-volume, high-resolution microscopy of diverse cell types in lymphoid and nonlymphoid organs, as well as to perform quantitative analysis of the composition and tissue distribution of multiple cell populations in lymphoid tissues. Combined with histo-cytometry, Ce3D provides a comprehensive strategy for volumetric quantitative imaging and analysis that bridges the gap between conventional section imaging and disassociation-based techniques.”

Ce3D imaging allows scientists to view multiple cell types, shown here marked with assorted colors, that make up complex tissues like this lymph node in three dimensions.

In order to find the correct combination for the Ce3D method, the research team screened different reagants, using the lymphoid tissues of mice, because the level of detail and complexity in the tissue is high enough for the researchers to “accurately interpret how this tissue responds to medication and immunotherapy.”

Once they had the proper combination, they also tested the method on tissue from the intestine, liver, lung, muscle, and thymus, and were able to get detailed, comprehensive 3D images of each.

The medical field, in terms of cancerous growths, is turning more and more frequently these days to responses that are tailored to individual tumors. NIAID’s new Ce3D method could prove very important for researchers who are working to evaluate therapies in the lab, and for doctors evaluating real patients, as it’s a very sophisticated technique to get detailed information about a tumor. In addition, the technique is more scalable and cost-effective than tissue clearing methods, as it uses existing laboratory instruments and less expensive substances. Discuss in the 3D Tissue Imaging forum at 3DPB.com.

[Source/Images: NIAID]

 

Facebook Comments

Share this Article


Related Articles

Interview with Len Wanger of Deer Valley Ventures

Malaria Diagnostics: Researcher 3D Prints Microscope Accessory for Imaging on Smartphones



Categories

3D Design

3D Printed Art

3D Printed Food

3D Printed Guns


You May Also Like

Lamar University Researchers Develop 3D Printed Self-Healing Material to Cut Back on Waste

Material sample with a healed break [Image: Dr. Keivan Davami] A team of researchers from Lamar University in Texas, led by assistant professor Dr. Keivan Davami, recently developed a self-healing...

University of Mississippi: How to Trace 3D Printed Guns for Forensic Analysis

Parker Riley Ball is a thesis student at the University of Mississippi, exploring some complex areas regarding 3D printing, outlined in ‘Development of a Dart-Mass Spectral Database for 3D Printed...

A Window Into the Brain: Transparent 3D Printed Mouse Skulls for Alzheimer’s Research

There’s a long way to go to understanding the brain. So many questions remain unanswered, yet so much research is being done, like the former U.S. administration’s ambitious project, which...

Switzerland: Exciting New Technology Multi-Metal Electrohydrodynamic Redox 3D Printing

Researchers from Switzerland explain more about how metals dissolved and re-deposited in liquid solvents can further AM processes by promoting fabrication without post-processing. Their findings are outlined in the recently...


Training


Shop

View our broad assortment of in house and third party products.


Print Services

Subscribe To Our Newsletter

Subscribe To Our Newsletter

Join our mailing list to receive the latest news and updates from our 3DPrint.com.

You have Successfully Subscribed!