Scientists from the Université de Montréal have published a study detailing the ability to bioprint adult brain cells. Key to the research was a new laser-assisted technology that made it possible to ensure high levels of cell viability and functionality.
The team calls the process “Laser-Induced Side Transfer (LIST)”, previously covered on 3DPrint.com here, and it has advantages over some other bioprinting techniques through its ability to print with materials of different viscosities. To demonstrate the potential of the technique in neurology, the researchers 3D printed sensory neurons, key to the peripheral nervous system and the ability to process sense stimuli.
The researchers suspended dorsal root ganglion (DRG) neurons, extracted from the peripheral nervous system of mice, within a bioink solution. This was loaded into a square capillary atop a biocompatible substrate before low-energy nanosecond lasers were pulsed as the middle fo the capillary. This resulted in the formation of microbubbles that, when expanding, ejected cells onto the below substrate. Next, the samples were incubated, washed, and then incubated once more for 48 hours.
Running a viability assay, the team determined that 86 percent of the cells were still viable two days after printing. These results were further improved when the lasers used lower amounts of energy, as higher power levels damaged the cells. Other tests were encouraging as well. These included calcium imaging, RNA sequencing, neuropeptide release, and measuring the ability to form new projections in response to environmental cues (neurite outgrowth).
It’s worth noting that this is not the first bioprinting method that involves the use of lasers, nor the first 3D printed adult braincells. Poietis, for instance, is known for its laser-assisted bioprinting technique, which relies on laser pulses to deposit cell-laden bioinks onto an ink film spread on a glass plate. Researchers in China were able to maintain bioprinted neuron viability for an impressive four weeks. Fluicell has bioprinted neural cells in the past. A University of Minnesota team was able to 3D print neurons to create a bioficial spinal cord.
The study suggests a number of possible applications, such as drug testing, disease modeling, and 3D printing implants. Most immediately, the team is considering the use of this technology for developing new medications.
Subscribe to Our Email Newsletter
Stay up-to-date on all the latest news from the 3D printing industry and receive information and offers from third party vendors.
You May Also Like
Formlabs Breaks New Ground with Alumina and Silicone 3D Printing
Formlabs announced a suite of major innovations at the 2023 Digital Factory Conference in Boston’s SoWa Power Station, with implications set to transform industries from automotive to healthcare. The company...
3D Printing Webinar and Event Roundup: September 10, 2023
This might possibly be the longest webinar and event roundup we’ve ever done at 3DPrint.com—that’s how many offerings there are this week! I won’t waste your time in this introduction...
3D Printing News Briefs, August 23, 2023: Sales & Distribution, Parametric Modeling, & More
In business news today, nano3Dprint announced its latest strategic distribution partnership in Asia, Snowbird Technologies is Meltio’s newest sales partner, and EOS North America launched its AMTurnkey Service. Continuing with...
3D Printing Webinar and Event Roundup: August 20, 2023
In this week’s roundup of 3D printing webinars and events, Stratasys continues its North American tour, and 3D Systems starts its Additive Insights Roadshow. There’s an Automation Expo in Mumbai,...
Upload your 3D Models and get them printed quickly and efficiently.