Additive Manufacturing Strategies

Bioprinting Biocompatible Hydrogels from Cellulose Inks

ST Medical Devices

Share this Article

Researchers from Italy and Sweden add to the ongoing trend for improving bioprinting techniques and materials. Upon developing bio-based photocurable materials for 3D printing and bioprinting with hydrogels, the authors released the details of their study in ‘DLP 3D Printing Meets Lignocellulosic Biopolymers: Carboxymethyl Cellulose Inks for 3D Biocompatible Hydrogels.’

Modified carboxymethyl cellulose was at the center of this experiment for bioprinting with digital light processing (DLP). While more commonly used as a filler, cellulose has been used in other inks. Beginning the research with the study of lignocellulosic biopolymers, the authors explained that they present a range of options for printing with DLP, while carboxymethyl cellulose (CMC) is often used in food, paint, and detergents. For this reason, it is a sustainable material with particular utility in bioprinting.

Approved by the FDA, and deemed biocompatible, CMC is water-soluble, versatile, and considered “an ideal candidate for the preparation of novel photocurable resins for DLP.” These types of formulations can also imitate cell microenvironments because of their similar makeup to glycosaminoglycan found within the extracellular matrix.

“Due to its versatility, its advantageous properties, water-solubility, and susceptibility to further functionalization, we also expected CMC would be an ideal candidate for the preparation of novel photocurable resins for DLP,” explained the authors. “However, the use of light-assisted printing techniques requires reactive photocrosslinkable functional groups, which means CMC needs functionalization to produce ink formulation for the production of 3D photocured hydrogels.

“CMC was therefore methacrylated and its photorheology and DLP printability was investigated in two formulations, namely, M-CMC/Dulbecco’s Modified Eagle Medium (DMEM) and M-CMC/water, in presence of a fixed amount of lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) photoinitiator.”

(A) Scheme for the methacrylation of carboxymethyl cellulose (CMC). The presented product only presents one of the possible reaction products. (B) FTIR and (C) 1H NMR spectra for methacrylated CMC (M-CMC, red) and neat CMC (CMC, black).

During evaluation, the authors also investigated compatibility for hydrogels, with M-CMC solubilized in a culture medium (DMEM). Rheological properties (storage modulus, G′, and loss modulus, G″) were evaluated during UV curing for CMC/DMEM/LAP and M-CMC/water/LAP:

“Although the formulation M-CMC/DMEM/LAP showed a slight delay with respect to the onset of the curing process, the DMEM medium still allowed sufficient light penetration for the photocuring process in view of 3D printing,” said the researchers.

Both the CMC/DMEM/LAP and M-CMC/water/LAP formulations proved to be stable after 90 s of UV irradiation. Hydrogels were created from both formulations, and deemed “extremely promising” in comparison with other DLP biocompatible materials.

(A) Photorheology of methacrylated carboxymethyl cellulose (M-CMC) 20 mg/mL (2 wt% lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP)) solubilized in water (black) or in culture medium (pink). (B) Gel point. Film thickness 300 m. (C) Frequency sweep. Strain rate 1% and oscillation frequency from 0.01 to 10 Hz.

The researchers created a variety of 3D printed samples, to include cylinders, parallelepipeds, and other complex structures—all stemming from the M-CMC/DMEM/LAP and M-CMC/water/LAP formulations. On further evaluation, the hydrogels were stable, flexible, and the photocrosslink reaction was completed. Although dyes can be helpful in limiting light diffusion, there is also the risk of cytotoxicity, leading the authors to avoid such use in this study.

3D printed M-CMC hydrogels. (A) Simple cylinders and parallelepipeds (solvent: water). (B) The hydrogel exhibited good flexibility and handleability. (C) SEM analysis performed on the freeze-dried hydrogel. (D–F) 3D objects printed from water (D) and from culture medium solution (E,F).

Crosslinking and reactivity were further evaluated, along with compression tests, assessment of swelling ability, and cytotoxicity testing to investigate lack of cell death due to release of LAP photoinitiator or unreacted polymer chains. Ultimately, the team of researchers reported that there were no signs of cytotoxicity, and overall, their work was successful with cells exhibiting viability similar to control samples.

3D-printed M-CMC hydrogels. (A) Simple cylinders and parallelepipeds (solvent: water). (B) The hydrogel exhibited good flexibility and handleability. (C) SEM analysis performed on the freeze-dried hydrogel. (D–F) 3D objects printed from water (D) and from culture medium solution (E,F).

[Source / Images: ‘DLP 3D Printing Meets Lignocellulosic Biopolymers: Carboxymethyl Cellulose Inks for 3D Biocompatible Hydrogels’]

Share this Article


Recent News

3D Printing News Briefs, January 22, 2022: Research, Business, & More

3D Printing Robots Receive €1 Million Boost



Categories

3D Design

3D Printed Art

3D Printed Food

3D Printed Guns


You May Also Like

Accelerate to AM Success with Simulation

The benefits of an additive manufacturing (AM) program are highly compelling –for the creation of highly complex parts, economically manufacturing lot sizes of one, and the near elimination of wasted...

2022 Predictions: 3D Printing for Series Production of Metal Parts

It’s time to gaze into the tea leaves and imagine a bright future for 3D printing. In this article, we will be looking specifically at predictions, trends, and developments in...

Featured

BMW Leads Seed Round for Rubber 3D Printing Startup Rapid Liquid Print

Boston 3D printing company Rapid Liquid Print (RLP) is working to make a new class of 3D printers that can effortlessly build large-scale, high-resolution, soft, and stretchable products using industry-grade...

3D Printing Webinar and Event Roundup: December 5, 2021

We’ve got another busy week of webinars and events to tell you about, with topics ranging from aviation and medical 3D printing to a town hall meeting, biomaterials, SLA technology,...


Shop

View our broad assortment of in house and third party products.