Additive Manufacturing Strategies

Antibacterial Properties & Cytocompatibility of EPL in 3D Printed Composite Scaffolds

ST Medical Devices

Share this Article

Chinese researchers continue to study 3D printing materials and structures, releasing their findings in the recently published ‘Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds.’

The study of biocompatible materials and scaffolds suitable for sustaining cells is of enormous interest to researchers around the world as they hope to move forward in tissue engineering—and of course eventually reaching the ultimate goal of 3D printing human organs and one day eliminating waiting lists for transplants. Today, bioprinting has offered strides in creating innovative microenvironments, seeding cells like human dermal fibroblasts, and even fabricating heart tissue in space.

Bone regeneration continues to be an area of challenge overall, but progress is being made with tissue engineering.

“Bone tissue engineering provides a new route for the therapy of bone defects. The scaffolds for bone tissue engineering must have pores interconnected in three dimensions, with highly regular pore formation and structure. The porous structure provides space for cell migration, adhesion, and the ingrowth of new bone tissue. Scaffolds for bone tissue engineering should have reasonable strength and bioactivity, without causing any adverse effects,” explained the researchers.

SEM images: PCL scaffold at 300 μm (a), PCL scaffold at 5 μm (b), PCL/HA scaffold at 300 μm (c), PCL/HA scaffold at 5 μm (d), EPL/PCL/HA scaffold at 300 μm (e), EPL/PCL/HA scaffold at 5 μm (f).

In attempting to create an aseptic environment, the researchers used ε-poly-L-lysine (EPL), an antimicrobic cationic polypeptide for modifying the surfaces of polycaprolactone/hydroxyapatite (PCL/HA) scaffolds. EPL, offering a “wide antimicrobial spectrum,” is already popular for use in food applications, as well as electronics, agriculture, and more; to date, it has not been used with 3D printed PCL/HA scaffolds.

“Microorganisms such as bacteria and fungi do not easily develop resistance to this polypeptide,” stated the researchers.

The mission in this study was to use FDM 3D printing to fabricate scaffolds and then analyze the properties of the ε-poly-lysine/polycaprolactone/hydroxyapatite. The team also examined both biocompatibility and osteoconductivity of the scaffolds.

Antibacterial activity was examined with the ‘zone of inhibition test,’ using the following:

  • aureus(Gram-positive bacteria)
  • coli(Gram-negative bacteria)
  • mutans(oral facultative anaerobic bacteria)

Upon magnifying the scaffolds, the researchers noted that pore sizes were ‘within range’ for suitable bone regeneration—leading to the conclusion that the scaffolds would be able to support the ‘formation and growth of bone.’ Further examination showed that EPL was present in scaffolds and its chemical properties did not change. EPL was found to offer great potential and versatility for improving the scaffolds in the initial stages of bone formation.

Antimicrobial effect of scaffolds on S. aureus (A), E. coli (B) and S. mutans (C); bacteria cultures after 24 hours: PCL scaffolds (Aa, Ba and Ca), PCL/HA scaffolds (Ab, Bb and Cb), EPL/PCL/HA scaffolds (Ac, Bc and Cc); subsequent culture for 3 days: PCL scaffolds (Ad, Bd and Cd), PCL/HA scaffolds (Ae, Be and Ce), EPL/PCL/HA scaffolds (Af, Bf and Cf).

“The created scaffolds were found to be cytocompatible as well as capable of osteogenic differentiation and antimicrobial activity in vitro, which is beneficial not only to bone regeneration, but to reduce or prevent the incidence of the infective complications in reparative bone formation,” concluded the researchers. “Further investigations are needed to determine if the present scaffolds can support functional tissue regeneration in vivo.”

What do you think of this news? Let us know your thoughts! Join the discussion of this and other 3D printing topics at 3DPrintBoard.com.

(A) Stress strain curves of PCL and PCL/HA scaffolds. (B) Compressive modulus of PCL and PCL/HA scaffolds. The data were represented as mean ± standard deviation (SD; n = 5; *p < 0.05).

[Source / Images: ‘Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds’]

Share this Article


Recent News

3D Printing Webinar and Event Roundup: January 23, 2022

3D Printing News Briefs, January 22, 2022: Research, Business, & More



Categories

3D Design

3D Printed Art

3D Printed Food

3D Printed Guns


You May Also Like

3D Printing News Briefs, January 15, 2022: 3D Laser Printing, Housing, & More

We’re starting with some interesting research in 3D Printing News Briefs today, which could help reduce the cost and size of 3D laser printing. Moving on, a cancer patient is...

3D Printed Vaginal Rings Could Treat Bacterial Infections

There are plenty of examples in which 3D printing has been used to develop drug delivery systems, but this research out of Hungary is tackling the issue from a new...

3D Printing News Briefs, January 12, 2022: Rebranding, Bioprinting, & More

First up in today’s 3D Printing News Briefs, Particle3D has gone through a rebrand, and a team of researchers developed a way to 3D print and preserve tissues in below-freezing...

3D Printing News Briefs, January 8, 2021: Business, Doxing, 3D Printed Lights, & More

We’re starting with business in today’s 3D Printing News Briefs, as RadTech announced new board members and Ziggzagg is investing in AM-Flow’s workflow automation technology. Cults3D was recently in hot...


Shop

View our broad assortment of in house and third party products.