Bone Regeneration: Successful Bioprinting with Poly-Lactic-Co-Glycolic Acid/β-Tricalcium Phosphate Scaffolds
In ‘Poly(Dopamine) Coating on 3D-Printed Poly-Lactic-Co-Glycolic Acid/β-Tricalcium Phosphate Scaffolds for Bone Tissue Engineering,’ researchers from the School of Stomatology at Jilin University in Changchun, China are seeking improved methods for treating bone defects caused by health issues like osteoporosis, malignant tumors, and physical trauma. While currently there are numerous limitations for medical treatment, bioprinting and bone tissue engineering show great potential.
For this study, the researchers 3D printed polydopamine-coated poly-(lactic-co-glycolic acid)/β-tricalcium phosphate composite scaffolds, followed by characterization and biocompatibility tests.
The sample scaffolds created by the research team exhibited a regular shape, and pore size of ~500 μm, with a honeycomb structure—a form not uncommon in bioprinting.
“The printed filaments are exhibited in a network structure, with a strong architectural integrity. The surface morphology of the fiber-aligned scaffolds, with or without PDA surface modification, can be characterized by SEM,” stated the researchers. “The results show that PDA0, PDA1, and PDA2 scaffolds have pores distributed over the internal walls, with a highly roughened structural coating layer and uneven micro-surface in comparison with non-coated scaffolds.”
PDA0, PDA1, and PDA2 were evaluated for surface wettability using water contact angle measurement, with PDA0 exhibiting the highest contact angle at 88.3°, 68.5°, and 55.1°, respectively (p < 0.05). Porosities were found to be 60.31%, 61.1%, and 59.67%, respectively. While porosity can be maintained during bone tissue engineering, density varies
For cell attachment tests, the research team used mouse pre-osteogenic cells, demonstrating cell seeding efficiencies of the PDA0, PDA1, and PDA2 scaffolds at 56%, 76%, and 82%, respectively. They also noted that the PDA2 group ranked highest in adhesion (p < 0.05). As the PDA coating increased, cell membranes spread more expansively.
Ultimately, the PDA-coated PLGA/β-TCP composite scaffolds were 3D printed successfully, according to the research team. Along with successful fabrication efforts, the researchers found that the sample scaffolds were ‘suitable’ as vehicles for bone tissue engineering. Using a one-way ANOVA analysis, the team evaluated the variations between the experimental groups. Results were ‘statistically significant’ at a p value <0.05.
“Furthermore, the in vitro cell biocompatibility test results show that the PDA coatings can improve cell adhesion and osteogenic differentiation. To further study the osteogenic effects of the PDA-coated scaffolds, we established a mouse skull defect model and implanted the scaffolds in the defects for a period,” concluded the authors.
“The bones with scaffolds were extracted to be analyzed for new bone formation, and the results show that the PDA-coated composite scaffolds perform a satisfactory repair effect. In conclusion, our results demonstrate that the simplified bio-inspired surface modification of PLGA/β-TCP scaffolds by PDA is a very promising method for effectively repairing bone defects.”
Because of the difficulties and challenges in bone tissue engineering, there is continued research into the subject, from studies regarding nanofiber tubular scaffolds to structures made with tricalcium phosphate, porous metallic biomaterials, and more.
What do you think of this news? Let us know your thoughts! Join the discussion of this and other 3D printing topics at 3DPrintBoard.com.[Source / Image: ‘Poly(Dopamine) Coating on 3D-Printed Poly-Lactic-Co-Glycolic Acid/β-Tricalcium Phosphate Scaffolds for Bone Tissue Engineering’]
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