AM Energy

Bioprinting 101: Part 5 – Bioink

Electronics
AMR Military

Share this Article

Extrusion of Bioink

Bioink refers to substances made of living cells that can be used for 3D printing of complex tissue models. Bioinks are materials that mimic an extracellular matrix environment to support the adhesion, proliferation, and differentiation of living cells. Bioinks distinguish themselves from traditional biomaterials such as hydrogels, polymer networks, and foam scaffolds due to their ability to be deposited as filaments during an additive manufacturing process. They can be alginate-based, gelatin based, pluronics, and decellularized ECM-based.

Alginate is a naturally derived biopolymer from the cell wall of brown algae that has been widely used as a biomaterial. Alginates are particularly suitable for bioprinting due to their mild cross-linking conditions via incorporation of divalent ions such as calcium. These materials have been adopted as bioinks through increasing their viscosity. Additionally, these alginate-based bioinks can be blended with other materials such as nanocellulose for application in tissues such  as cartilage.

Gelatin has been widely utilized as a biomaterial for engineered tissues. The formation of gelatin scaffolds is dictated by the physical chain entanglements of the material which forms a gel at low temperatures. However, at physiological temperatures (body temperature), the viscosity of gelatin drops significantly. Methacrylation of gelatin is a common approach for the fabrication of gelatin scaffolds that can be printed and maintain shape fidelity at physiological temperature. Methacrylation is important for restorative material properties and bioengineering.

Decellularized Extracellular Matrix

 

Decellularized extracellular matrix based bioinks can be derived from almost any mammalian tissue. However, most organs such as heart, muscle, cartilage, bone, and fat are decellularized, lyophilized, and pulverized, to create a soluble matrix that can then be formed into gels. These bioinks have advantages over other materials due to their derivation from mature tissue. These materials consist of a complex mixture of decellularized extracellular matrix and proteins specific to their tissue origin. Therefore, dECM-derived bioinks are particularly tailored to provide tissue-specific cues to cells. Often these bioinks are cross-linked through thermal gelation or chemical cross-linking such as through the use of riboflavin.

Pluronics have been utilized in printing application due to their unique gelation properties. Below physiological temperatures, the pluronics exhibit low viscosity. However, at physiological temperatures, the pluronics form a gel. A more permanent pluronic-based network can be formed through the modification of the pluronic chain with acrylate groups that may be chemically cross-linked.

Pluronic

So, I have done my obligatory duty of informing you with new material. However, I am not here to only be a scribe with no life. Let us get a little more excited about this article. Is it not wild to think that most of the materials used in bioinks are readily synthesizable? I could order all of this and make some in my own home if I wanted. I also think the material properties discussed are so vast and interesting. Just having a little bit of organic chemistry as well as biochemistry knowledge leads a user to fully enjoy and soak in the importance of biomaterials. I believe it is necessary for me to at some point show some live demonstrations of synthesis with these materials. Talking gets boring after a while, but practical work gets exciting.

Share this Article


Recent News

Authentise Weaves 3D Printing’s Digital Thread with Trio of New Government Grants

New Spanish Project to Create Recycled Polypropylene Powder for 3D Printing



Categories

3D Design

3D Printed Art

3D Printed Food

3D Printed Guns


You May Also Like

3D Printing News Briefs, July 19, 2023: Local Motors IP Acquisition, Pizza Delivery, & More

We’re starting with materials in today’s 3D Printing News Briefs, as parts made with Arkema’s bio-based Pebax(R) Rnew(R) elastomeric materials are now available from Erpro 3D Factory. On to business...

Xtellar: Braskem Spins off 3D Printing Materials Division into New Business

With $14 billion in revenues, Brazil’s Braskem is the largest petrochemical company in Latin America and the largest polyolefins producer in the Americas, particularly polypropylene (PP). The giant has taken...

Filtration Leader 3D Prints Critical Wastewater Screens with HP’s MJF

As additive manufacturing (AM) makes its way from aerospace into general industry and society at large, the flow may begin to shift from a trickle to a raging rapid. A...

3D Printing News Unpeeled: Dior, Botter and Rains 3D Print Shoes at Paris Fashion Week

Danish brand Rains has made a 3D printed TPU shoe together with Zellerfeld. Zellerfeld is a shoe 3D Printing service. Meanwhile Dior made a powder bed fusion cellular shoe out...