3D Printed Device Allows Living Biosensor to be Deployed in the Field

Share this Article

Genetically modified organisms, or GMOs, are subject to strict regulation. In a paper entitled “An Additive Manufacturing Approach that Enables the Field Deployment of Synthetic Biosensors,” a group of researchers explains that living biosensors, which can be deployed for disease diagnosis in the field, are considered GMOs and are therefore subject to that same regulation. Using 3D printing is one way in which these biosensors can be made secure and acceptable for deployment in the field.

In rural and less-developed areas, the researchers explain, the pathogen P. aeruginosa can be acquired in local clinics. The organism is found in lung sputum samples upon infection, and the samples can be collected and placed in commercially available sample tubes. The researchers’ study focuses on creating a biosensor that could be used to immediately diagnose the disease in the field. They used E. coli as a biosensor, but needed a way to safely transport the dangerous bacteria from the laboratory. So they 3D printed a unique cap that completely encases the bacteria and can be securely sealed onto a sample tube containing lung sputum samples.

When the sample is ready to be analyzed, force is applied with the user’s finger to the cap, which causes the E. coli biosensor to break out of its 3D printed housing and drop into the sample.

“Detection of 3O-C12, which will be produced by P. aeruginosa in the sample or is already present in the sample, causes the living biosensor to produce mCherry and turns the sample red; this color can be detected through fluorescence analysis or visually with the human eye,” the researchers explain. “Thus, a colorimetric change in the sample can indicate the presence of P. aeruginosa in the lungs.”

P. aeruginosa, they continue, functions as an opportunistic pathogen in the lungs of patients with cystic fibrosis. Currently, it is diagnosed either with enzyme-linked immunosorbent assays (ELISAs) and real-time polymerase chain reaction (PCR) assays, which are either expensive or require extensive training to administer. The researchers’ hope is that their biosensor, with its secure 3D printed containment device, can be used as an inexpensive, easy-to-use alternative.

The 3D printed device was designed in Autodesk Inventor and 3D printed in ABS ULTRAT using a Zortrax M200 3D printer.

“To encase the 3O-C12 living biosensor into the 3D-printed device, a 40 µL agar plug was added to the bacterial well in the housing; the engineered E. coli cells were then plated onto the agar surface,” the researchers state. “After overnight culture, the plunger was pressed into the housing and a press-fit seal was formed to isolate the bacterial cells from the environment. The cap containing the living biosensor could then be safely transferred to the field and stored in a refrigerator for up to 30 d before use.”

The biosensor quickly senses the presence of the P. aeruginosa pathogen, and because of its enclosure within the 3D printed device, it should face far fewer regulatory hurdles, allowing it to be deployed freely outside the lab.

“The 3D-printed device turns the GMO into a component of the overall device and ensures that the biosensor is isolated from the environment,” the researchers conclude. “Thus, 3D printing this device enables our living biosensor, with its versatility to process environmental input, to be field-deployed as a novel, cost-effective diagnostic technology.”

Authors of the paper include Daniel Wolozny, John R. Lake, Paul G. Movizzo, Zhicheng Long and Warren C. Ruder.

Discuss this and other 3D printing topics at 3DPrintBoard.com or share your thoughts below.

 

Share this Article


Recent News

What is Metrology Part 21 – Getting Started with Processing

Analyzing & Solving 3D Printing Issues with Microfluidics



Categories

3D Design

3D Printed Art

3D Printed Food

3D Printed Guns


You May Also Like

Multimaterial 3D Printing Filaments for Optoelectronics

Authors Gabriel Loke, Rodger Yuan, Michael Rein, Tural Khudiyev, Yash Jain, John Joannopoulous, and Yoel Fink have all come together to explore new filament options, with their findings outlined in...

Germany: Two-Photon Polymerization 3D Printing with a Microchip Laser

Laser additive manufacturing technology is growing more prevalent around the world for industrial uses, leading researchers to investigate further in relation to polymerization, with findings outlined in the recently published...

3D Printing Polymer-Bonded Magnets Rival Conventional Counterparts

Authors Alan Shen, Xiaoguang Peng, Callum P. Bailey, Sameh Dardona, and W.K Anson explore new techniques in ‘3Dprinting of polymer-bonded magnets from highly concentrated, plate-like particle suspension.’ While magnets have...

South Africa: FEA & Compression Testing of 3D Printed Models

Researchers D.W. Abbot, D.V.V. Kallon, C. Anghel, and P. Dube delve into complex analysis and testing in the ‘Finite Element Analysis of 3D Printed Model via Compression Tests.’ For this...


Shop

View our broad assortment of in house and third party products.


Print Services

Subscribe To Our Newsletter

Subscribe To Our Newsletter

Join our mailing list to receive the latest news and updates from our 3DPrint.com.

You have Successfully Subscribed!