Nanjing Medical University: 3D Printing Scaffolds with Lithium for Cartilage Regeneration
In ‘Lithium Chloride-Releasing 3D Printed Scaffold for Enhanced Cartilage Regeneration,’ researchers from Nanjing Medical University experiment with coated scaffolds for furthering cell regeneration in patients. The authors realized the importance of such a study due to the challenges often encountered in renewing cartilage after physical damage.
While lithium is well-known for controlling bipolar disorder, previous research has shown that it may influence lessening the effects of arthritis, as well as preventing cartilage degradation in osteoarthritis. Here, the researchers investigated the use of lithium in creating poly-e-caprolactone (PCL) scaffolds, and then refining them further into a PCL-PDA-Li scaffold – (where the PDA as an abbreviation for polydopamine).
The authors then continued to evaluate the structures for:
- Physiochemical properties
They also compared them to PCL scaffolds and PCL-PDA scaffolds upon 3D printing a range of samples. While the pure PCL scaffolds were white (as in the normal color of PCL), PCL-PDA and PCL-PDA-Li scaffolds were dark brown after the researchers applied the PDA coating. The addition of lithium did not change coloration at all in scaffolding. All three scaffolds were smooth, but there were differences in the PCL-PDA scaffolds with small granules, and LiCl crystals attached to the granules in the PCL-PDA-Li scaffolds.
The researchers stated that hydrophilic scaffolds like PCL-PDA and PCL-PDA-Li scaffolds can provide better adherence of cells. There were no obvious disparities between the samples in mechanical properties within this research study. Ultimately, however, the researchers stated that they still found the lithium to be lacking in the desired effect for scaffolding and consequent tissue regeneration.
“Improvement of scaffolds is needed to provide a better environment for chondrogenesis,” stated the researchers. “Due to the poor expansion capacity, chondrocytes are not an ideal choice for cartilage tissue engineering. BMSCs have an extraordinary potential for proliferation and multipotential differentiation, including chondrogenesis, can be easily harvested by bone marrow.”
Overall upon comparing all the samples, the researchers discovered that PCL has the best strength and biodegradation, making it more suitable for scaffolds.
“We successfully synthesized PCL-PDA-Li scaffolds by 3D printing following a simple 2-step method. PDA coating and LiCl deposition improves surface hydrophilicity without sacrificing mechanical strength. In vitro experiments showed that PCLPDA-Li scaffolds promote better chondrocyte adhesion and cartilage matrix deposition. Our results demonstrate a better method for lithium administration and present a promising scaffold for cartilage tissue engineering,” concluded the researchers.
Tissue engineering today allows for the creation of new cells and structures able to heal patients in need; regeneration of cartilage is one area that is challenging, along with bone regeneration, skin grafts, and scaffolding for breast reconstruction. Find out more about 3D printed scaffolds for enhanced cartilage regeneration here. What do you think of this news? Let us know your thoughts! Join the discussion of this and other 3D printing topics at 3DPrintBoard.com.[Source / Images: Lithium Chloride-Releasing 3D Printed Scaffold for Enhanced Cartilage Regeneration]
You May Also Like
Electrospinning Fibers for Better Success in 3D Printing Biomimetic Scaffolds
In ‘Three-dimensional printed electrospun fiber-based scaffold for cartilage regeneration,’ Chinese researchers attempt to improve on processes for creating better materials and customized geometries in bioprinting. Here, they not only employ...
Tessenderlo Group Releases First Gelatin Bioink in Claro Series
An inclusive team of scientists at Tessenderlo Group, headquartered in Belgium, has announced a recent breakthrough in bioprinting with the creation of new gelatin materials for tissue engineering. Today, they...
Are Bioprinting Proponents Just Big Talkers?
In the paper “Three‐Dimensional Bioprinting in Regenerative Medicine: Reality, Hype, and Future,” authors Anthony Atala and Gabor Forgacs explore some necessary topics within 3D printing and bioprinting. The technology has...
Bioprinting for Bone Regeneration with Nanofiber Coated Tubular Scaffolds
Researchers from both Mexico and Costa Rica have joined efforts to further research into bone regeneration via bioprinting, allowing doctors and surgeons to create patient-specific scaffolds for improved treatment. 3D...
View our broad assortment of in house and third party products.