AMS Spring 2023

Creating Living Materials Using Bacteria and 3D Printing

Inkbit

Share this Article

In a paper entitled “Programmable and printable Bacillus subtilis biofilms as engineered living materials,” a team of researchers discusses how they used 3D printing to produce custom nanoscale biomaterials from the natural secretion of amyloid fibers from the bacteria Bacillus subtilis. The bacteria generate biofilms by secreting amyloid fibers via a tightly controlled cluster of genes called the tapA-sipW-tasA operon. TapA nucleates the extracellular assembly of TasA proteins to create the amyloid nanofibers that give the biofilm its structural integrity. The researchers were able to genetically modify the TasA protein and introduce functional chemical groups onto the TasA fibers excreted by the bacteria. This means that the bacterial films could be designed to act as functional living materials.

The researchers were able to engineer the bacteria to secrete fibers containing enzymatic functional groups into harmless products. They also combined the biofilms produced with multiple bacterial strains, which allowed them to perform a two-step degradation of the pesticide paraoxan. This shows the potential for producing efficient, eco-friendly materials.

In addition to showing the functional capabilities of the biofilms, the researchers also studied their processability as materials. Because of the viscoelastic properties of the biofilms, they are well-suited to 3D printing. Modifying the functional groups on the secreted enzymes did not hinder the processability of the biofilms, instead enabling the researchers to tune their viscoelastic properties for 3D printing applications.

“In a series of increasingly complex proof-of-concept demonstrations, we deployed these engineered biofilms in fluorescence  detection, conjugation chemistry, single-substrate bioremediation, and multireaction bioremediation cascades incorporating NPs,” the researchers state. 
“We also exploited the intrinsic viscoelastic properties of our engineered biofilms and fabricated well-defined ‘living shapes’, trappinthese materials into hydrogels and microgels using 3D printing and microencapsulation techniques.”

The living materials were shown to be able to self-regenerate after printing, sustaining their original printing shape as well as their viscoelastic and functional properties. The bacteria were able to incorporate onto their fibers without affecting the biofilm growth or cell viability. The cells remained viable for five weeks without supplemental nutrition.

“As this new type of living functional material offers previously unattainable material performance properties relevant to manufacturing, our study opens the door for the development of many conceivable new classes of complex multifunctional materials and dynamic and regenerative nanotechnologies,” the researchers conclude.

(L to R) Suying Liu, Chao Zhong, Jiaofang Huang and Chen Zhang

Authors of the paper include Jiaofang Huang, Suying Liu, Chen Zhang, Xinyu Wang, Jiahua Pu, Fang Ba, Shuai Xue, Haifeng Ye, Tianxin Zhao, Ke Li, Yanyi Wang, Jicong Zhang, Lihua Wang, Chunhai Fan, Timothy K. Lu and Chao Zhong.

Discuss this and other 3D printing topics at 3DPrintBoard.com or share your thoughts below.

 

Share this Article


Recent News

18 Lasers Power SpaceX Alums’ New Metal 3D Printing Tech

3D Printing News Unpeeled: Lamps from Fishing nets and a 3D Printed Flight Simulator



Categories

3D Design

3D Printed Art

3D Printed Food

3D Printed Guns


You May Also Like

C3Nano Launches “First” Low-Temperature Conductive Ink for Electronics 3D Printing

C3Nano, a Silicon Valley-based additive manufacturing (AM) materials company that specializes in conductive inks, announced the release of SuperGrid: a material that is pitched as “the first low-temperature curing,” flexible...

3D Printing News Unpeeled: Polymers with programable degradation, four story buildings and Hypersonics

The Growing Additive Manufacturing Maturity for Airbreathing Hypersonics, or GAMMA-H project shows that the US government is serious about hypersonics. Meanwhile CyBe wants to 3D print a four story building...

Furniture-Maker Launches First 3D Printed Lighting Collection from Sustainable Materials

Model No., started in Oakland, CA, in 2018, is a furniture manufacturer that uses PLA pellets derived from agricultural waste to 3D print made-to-order home furnishings. Model No.’s latest product...

3D Printing News Briefs, January 8, 2021: Business, Doxing, 3D Printed Lights, & More

We’re starting with business in today’s 3D Printing News Briefs, as RadTech announced new board members and Ziggzagg is investing in AM-Flow’s workflow automation technology. Cults3D was recently in hot...